The Lipid Raft-Associated Protein CD98 Is Required for Vaccinia Virus Endocytosis

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The lipid raft-associated protein CD98 is required for vaccinia virus endocytosis.

Mature vaccinia virus (vaccinia MV) infects a broad range of animals in vivo and cell cultures in vitro; however, the cellular receptors that determine vaccinia MV tropism and entry pathways are poorly characterized. Here, we performed quantitative proteomic analyses of lipid raft-associated proteins upon vaccinia MV entry into HeLa cells. We found that a type II membrane glycoprotein, CD98, is...

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The Lipid Raft - Associated Protein CD 98 is Required 1 for Vaccinia Virus Endocytosis

3 4 Nina Schroeder, Che-Sheng Chung, Chein-Hung Chen, Chung-Lin Liao and Wen Chang 5 6 Institute of Molecular Biology, Academia Sinica, 128 Academia Road, Sec. 2, Nangang, 7 Taipei 11529, Taiwan, Republic of China 8 2 The Genomics Research Center, Academia Sinica, 128 Academia Road, Sec. 2, Nangang, 9 Taipei 11529, Taiwan, Republic of China 10 11 12 Running title: Role of CD98 in vaccinia virus...

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Clathrin-mediated endocytosis of a lipid-raft-associated protein is mediated through a dual tyrosine motif.

We have previously shown that the integral membrane protein CD317 has both a conventional transmembrane domain near its N-terminus and a C-terminal glycosyl-phosphatidylinositol (GPI) anchor. With the possible exception of a minor topological variant of the prion protein, there remain no other convincing examples of a mammalian protein with such a topology. CD317 is localised to cholesterol-ric...

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The vaccinia virus-stimulated mitogen-activated protein kinase (MAPK) pathway is required for virus multiplication.

Early events play a decisive role in virus multiplication. We have shown previously that activation of MAPK/ERK1/2 (mitogen-activated protein kinase/extracellular-signal-regulated kinase 1/2) and protein kinase A are pivotal for vaccinia virus (VV) multiplication [de Magalhães, Andrade, Silva, Sousa, Ropert, Ferreira, Kroon, Gazzinelli and Bonjardim (2001) J. Biol. Chem. 276, 38353-38360]. In t...

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Lipid raft-associated protein sorting in exosomes.

Exosomes are small membrane vesicles secreted by cells upon fusion of multivesicular endosomes with the cell surface. The mechanisms underlying the specific sorting of proteins in exosomal membranes are far from being unraveled. We demonstrate here, using different cells, that some molecules are released in the extracellular medium via their association with lipid raft domains of the exosomal m...

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ژورنال

عنوان ژورنال: Journal of Virology

سال: 2012

ISSN: 0022-538X

DOI: 10.1128/jvi.06610-11